Xanthomonas vs Caulobacter
Contributors
Researchers:
Description
Technical info
Tilt Series Date: 2017-03-09
Data Taken By: Debnath Ghosal
Species / Specimen: Xanthomonas citri
Strain: pv citri 306
Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 2°, constant angular increment, dosage: 160.0 eV/Ų, defocus: -8.0 μm, magnification: 27500x.
Microscope: Caltech Polara
Acquisition Software: UCSFTomo
Upload Method: pipeline
Processing Software Used: Raptor
Collaborators and Roles: Chuck Farah lab made the strains, data collected by Debnath Ghosal.
Purification / Growth Conditions / Treatment: 4 µL mixture of Xac and Caulobacter cells were pipetted on top of Au-Finder grids. These grids were glow discharged in advance and kept on top of Agar pads. Carbon side up. These were then kept at the BSL-II hood and left for about 10 min before freezing.
Sample Preparation: Freezing conditions: Blot force 6, wait time=0, drain time =1 sec, blot time= 10 sec
Attached Files
Xac_Cc_20170309a0046.mrc, Tilt Series (Pixel Size 0.39 nm), 882.9 MB
Xac_Cc_20170309a0046.rec, Reconstruction (Pixel Size 1.56 nm), 889.0 MB
Files
keyimg_dga2017-03-09-48.jpg
Additional details
Identifiers
- DOI
- 10.22002/gkn6a-0ak32
- TILT_SERIES_ID
- dga2017-03-09-48
Funding
- NIH
- HHMI
- Beckman Institute
- Gordon and Betty Moore Foundation
- Agouron Institute
- John Templeton Foundation
Dates
- Collected
-
2017-03-09
- Created
-
2017-03-10
- Updated
-
2017-04-07