Caltech Tomography Archive
Published 2011 | Version v1
Dataset Open

HUVECs-pNLGFP-VPS4DN

Creators

  • 1. ROR icon California Institute of Technology

Description

HUVECs cells were first transfected with VPS4DN construct using Nucleofector Kit. 4.5 h post-transfection cells were infected with pNLGFP-VSVG by spinoculation and then frozen 24 h post infection. Sample# C68

Technical info

Tilt Series Date: 2011-04-14

Data Taken By: Cora Woodward

Species / Specimen: HUVECs

Strain:

Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 150.0 eV/Ų, defocus: -10.0 μm, magnification: 22500x.

Acquisition Software: leginon

Upload Method: rundir

Processing Software Used: imod

Collaborators and Roles: VPS4DN construct provided by Wes Sundquist. pNL4GFP provirus construct from Irvin Chen (UCLA). Cells transfected, infected, frozen and imaged at Caltech by Cora Woodward

Purification / Growth Conditions / Treatment: whole cells grown on grids, transfected and then infected

Sample Preparation: manual blotting

Attached Files

11apr14b_C68-3_013_full.rec, Reconstruction (Pixel Size 2.0 nm), 734.0 MB

11apr14b_C68-3_013.st, Tilt Series (Pixel Size 0.5 nm), 1.0 GB

Files

keyimg_cwl2011-04-14-8.jpg

Files (1.8 GB)

Name Size
1.0 GB (external) Download
734.0 MB (external) Download
md5:2207c24b57684020dd9b14bc345f528c
1.2 MB Preview Download
md5:3e05ff971f7ecb6b4f1433f9dc54475b
12.3 MB Preview Download

Additional details

Identifiers

DOI
10.22002/gmz7x-m9f03
TILT_SERIES_ID
cwl2011-04-14-8

Funding

NIH
HHMI
Beckman Institute
Gordon and Betty Moore Foundation
Agouron Institute
John Templeton Foundation

Dates

Collected
2011-04-14
Created
2015-09-16
Updated
2015-09-16