Caltech Tomography Archive
Published 2010 | Version v1
Dataset Open

Skinny E. coli with GFP-MreB

Li, Zhuo1
  • 1. ROR icon California Institute of Technology

Description

Raw data files of Skinny E. coli with GFP-MreB

Files

10mar05a_ZL001_033low.jpg
Files (7.0 MB)
Name Size
10mar05a_ZL001_033low.jpg
md5:02202c204b1f42419381e4860f0abbc9
4.2 MB Preview Download
keyimg_zla2010-03-05-33.jpg
md5:6c858c1079962290275abf5f045685e9
2.8 MB Preview Download

Technical info

Tilt Series Date: 2010-03-05

Data Taken By: Zhuo Li

Species / Specimen: Escherichia coli

Strain:

Tilt Series Settings: Single Axis, tilt range: (-63.0°, 63.0°), step: 1.5°, constant angular increment, dosage: 190.0 eV/Ų, defocus: -12.0 μm, magnification: 18000x.

Acquisition Software: Leginon

Upload Method: webload

Processing Software Used: RAPTOR and Peach

Collaborators and Roles: Skinny E. coli (DS664) and plasmids pDS176 (gfp-mreB in pKG116) and pDS728 (gfp-mreB-A125V in pKG116) were provided by Daisuke Shiomi. Plasmids were transformed into DS664 by electroporation by Zhuo Li. Data collected and processed by ZL.

Purification / Growth Conditions / Treatment: L broth. GFP-MreB was induced by salicylate. For correlative LM and ECT, membrane dye FM4-64 was added (001-012)

Sample Preparation: R2/2 CF or Quantifoil, 10 nm gold, 100% humidity

External Files

Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/zla2010-03-05-33/rawdata/

10mar05a_ZL001_033.mrc, Tilt Series (Pixel Size 1.192 nm), 721.4 MB Download

Additional details

Identifiers Funding Dates
Created:
May 17, 2023
Modified:
August 29, 2025