Myxococcus xanthus PilO-sfGFP
Contributors
Researcher:
Description
Technical info
Tilt Series Date: 2014-03-10
Data Taken By: Yi-Wei Chang
Species / Specimen: Myxococcus xanthus
Strain:
Tilt Series Settings: Single Axis, tilt range: (-65.0°, 65.0°), step: 1.0°, constant angular increment, dosage: 200.0 eV/Ų, defocus: -8.0 μm, magnification: 27500x.
Microscope: Caltech Polara
Acquisition Software: UCSFTomo
Upload Method: pipeline
Processing Software Used: Raptor
Collaborators and Roles: Strain form Lotte Sogaard-Andersen
Purification / Growth Conditions / Treatment: Cells grown 48 hr in shaking CTT medium at 30 degrees without antibiotics. OD600=0.8
Sample Preparation: Vitrobot settings: 100% humidity, 3µl sample, 10 mg/ml BSA treated gold. -3.5 offset, 4 sec blot time, no drain. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200.
Attached Files
20140310_YWC092_20013.mrc, Tilt Series (Pixel Size 3.9 nm), 3.7 GB
20140310_YWC092_20013_part131_10.rec, Reconstruction (Pixel Size 15.6 nm), 712.7 MB
Files
keyimg_ycw2014-03-10-49.jpg
Additional details
Identifiers
- DOI
- 10.22002/gq0h4-xch21
- TILT_SERIES_ID
- ycw2014-03-10-49
Funding
- NIH
- HHMI
- Beckman Institute
- Gordon and Betty Moore Foundation
- Agouron Institute
- John Templeton Foundation
Dates
- Collected
-
2014-03-10
- Created
-
2014-03-12
- Updated
-
2014-03-17