Caltech Tomography Archive
Published 2015 | Version v1
Dataset Open

Dflg

Subramanian, Poorna1
  • 1. ROR icon California Institute of Technology
Researcher:
Sahand, Moh

Description

This is a flagellum mutant from Sahand (ATP connected) grown in serum bottle. We saw with perfusion setup that the same mutant makes nanowires. So I went back and imaged the serum bottle version just to make sure that it doesnt have nanowires.

Files

keyimg_psa2015-08-20-30.jpg
Files (16.2 MB)
Name Size
keymov_psa2015-08-20-30.mp4
md5:74d9811ef76661482ee9b8155c36ff7a
15.2 MB Download
keyimg_psa2015-08-20-30.jpg
md5:18d2aaa6cb5562386db968edb10bb498
1.0 MB Preview Download

Technical info

Tilt Series Date: 2015-08-20

Data Taken By: Poorna Subramanian

Species / Specimen: Shewanella oneidensis

Strain: MR-1

Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 2.0°, constant angular increment, dosage: 150.0 eV/Ų, defocus: -8.0 μm, magnification: 18000x.

Microscope: Caltech Polara

Acquisition Software: UCSFTomo

Upload Method: pipeline

Processing Software Used: Raptor

Collaborators and Roles: Moh and Sahand

Sample Preparation: same as before

External Files

Files available via S3 at ://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/psa2015-08-20-30/3dimage_60976/

dflgwithnanowires_atlas10030_part61_4.rec, Reconstruction (Pixel Size 2.4 nm), 445.4 MB Download

dflgwithnanowires_atlas10030.mrc, Tilt Series (Pixel Size 0.6 nm), 1.7 GB Download

Additional details

Identifiers Funding Dates
Created:
June 1, 2023
Modified:
August 28, 2025