Published 2013 | Version v1
Dataset Open

Mycobacter smegmatis pneumonia

  • 1. ROR icon California Institute of Technology

Contributors

Description

KP3 WT (S) M. smegmatis mc2_155

Technical info

Tilt Series Date: 2013-09-09

Data Taken By: Alasdair McDowall

Species / Specimen: Mycobacterium smegmatis

Strain: mc2_155

Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 180.0 eV/Ų, defocus: -8.0 μm, magnification: 27500x.

Microscope: Caltech Polara

Acquisition Software: UCSFTomo

Upload Method: pipeline

Processing Software Used: UCSF Tomo gatan K2 Raptor

Collaborators and Roles: Koushik Paul / Eric Brown Genentech, Inc. 1 DNA Way South San Francisco, CA 94080 (650) 225-6248 Fax: (650) 225-1107

Purification / Growth Conditions / Treatment: Saunton Media (S)

Sample Preparation: 3ul + 10nm Au R2/2 quantifoils 4.5 offset 1 blot 2s 1s drainsingle tilt. constant angular increment, step: 1. tilt range: (-60, 60). dosage: 180/A2. defocus: -8um. magnification: 26000

Attached Files

atlas10003.mrc, Tilt Series (Pixel Size 0.39 nm), 3.4 GB

atlas10003_full.rec, Reconstruction (Pixel Size 1.56 nm), 445.4 MB

Files

keyimg_amw2013-09-09-3.jpg

Files (3.9 GB)

Name Size
3.4 GB (external) Download
445.4 MB (external) Download
md5:dffbd5c4580c1ffa9e6f76c51a659c02
970.6 kB Preview Download
md5:fb71862239364c5d2b64ccf4079ed5f1
13.0 MB Preview Download

Additional details

Identifiers

DOI
10.22002/gy7s4-3ed57
TILT_SERIES_ID
amw2013-09-09-3

Funding

NIH
HHMI
Beckman Institute
Gordon and Betty Moore Foundation
Agouron Institute
John Templeton Foundation

Dates

Collected
2013-09-09
Created
2013-09-09
Updated
2013-09-11