Bdellovibrio attack-phase
Contributors
Researchers:
Description
Technical info
Tilt Series Date: 2013-08-20
Data Taken By: Yi-Wei Chang
Species / Specimen: Bdellovibrio bacteriovorus
Strain: HD100
Tilt Series Settings: Single Axis, tilt range: (-65.0°, 65.0°), step: 1.0°, constant angular increment, dosage: 100.0 eV/Ų, defocus: -6.0 μm, magnification: 27500x.
Microscope: Caltech Polara
Acquisition Software: UCSFTomo
Upload Method: pipeline
Processing Software Used: Raptor
Collaborators and Roles: Strain from Liz Sockett, Cells prepared by Lee Rettberg
Purification / Growth Conditions / Treatment: Cells grown in S17-1 prey solution at 30 degrees, OD600=0.2, filtered by 0.22 um filter, mixed with purified WM3433 mini E. coli for 10 min before freezing
Sample Preparation: Vitrobot settings: 100% humidity, 3µl sample, 10 nm BSA treated gold. -3 offset, 4 sec blot time, no drain. Ethane/propane mix. Quantifoil Cu Finder grid
Attached Files
20130820_Bdellovibrio+WM3433_10043.mrc, Tilt Series (Pixel Size 0.39 nm), 3.7 GB
20130820_Bdellovibrio+WM3433_10043_part131_2.rec, Reconstruction (Pixel Size 1.56 nm), 534.5 MB
Files
keyimg_ycw2013-08-20-52.jpg
Additional details
Identifiers
- DOI
- 10.22002/h21p3-z2v79
- TILT_SERIES_ID
- ycw2013-08-20-52
Funding
- NIH
- HHMI
- Beckman Institute
- Gordon and Betty Moore Foundation
- Agouron Institute
- John Templeton Foundation
Dates
- Collected
-
2013-08-20
- Created
-
2013-08-22
- Updated
-
2013-08-29