Myxococcus xanthus delta-pilM

Description

Technical info

Tilt Series Date: 2013-12-17

Data Taken By: Yi-Wei Chang

Species / Specimen: Myxococcus xanthus

Strain:

Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 200.0 eV/Ų, defocus: -10.0 μm, magnification: 34000x.

Microscope: Caltech Polara

Acquisition Software: Undefined

Upload Method: pipeline

Processing Software Used: Raptor

Collaborators and Roles: Strain form Lotte Sogaard-Andersen

Purification / Growth Conditions / Treatment: Cells grown 48 hr in CTT medium at 30 degrees without antibiotics. OD600=0.7

Sample Preparation: Vitrobot settings: 100% humidity, 3µl sample, 10 mg/ml BSA treated gold. -4 offset, 4 sec blot time, 1s drain. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200. 20 nm gold beads.

Attached Files

20131217_YWC058_10013.mrc, Tilt Series (Pixel Size 0.32 nm), 3.4 GB

20131217_YWC058_10013_full.rec, Reconstruction (Pixel Size 1.28 nm), 712.7 MB