Caltech Tomography Archive
Published 2011 | Version v1
Dataset Open

Cryo-sectioned Jurkat VSVG-pNL4GFP

Creators

  • 1. ROR icon California Institute of Technology

Contributors

Description

Jurkats infected by spinoculation with VSVG-pNL4GFP, collected 30 min after synchronous entry, HP frozen and cryo-sectioned by Mark Ladinsky. Used for CLEM

Technical info

Tilt Series Date: 2011-12-15

Data Taken By: Cora Woodward

Species / Specimen: Jurkat Human T-cell

Strain: Clone E6-1

Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 150.0 eV/Ų, defocus: -6.0 μm, magnification: 22500x.

Acquisition Software: UCSF tomo

Upload Method: rundir

Processing Software Used: imod

Collaborators and Roles: pNL4GFP provirus construct from Irvin Chen (UCLA). Vpr-gfp construct to label virus provided by Tom Hope. Virus made at Caltech by Cora Woodward

Purification / Growth Conditions / Treatment: infected VSVG-pNL4GFP

Sample Preparation: cryo-sectioned by Mark Ladinsky

11dec15_CRYOSECS_pNLEGFP_grid2_0005_ns_full.jpg: file associated to 3D Image #54418

Attached Files

11dec15_CRYOSECS_pNLEGFP_grid2_0005_ns.st, Tilt Series (Pixel Size 0.5 nm), 941.4 MB

11dec15_CRYOSECS_pNLEGFP_grid2_0005_ns_full.rec, Reconstruction (Pixel Size 2.0 nm), 445.9 MB

11dec15_CRYOSECS_pNLEGFP_grid2_0005_ns_full.tiff, file associated to 3D Image #54418, 2.1 MB

Files

11dec15_CRYOSECS_pNLEGFP_grid2_0005_ns_full.jpg

Files (1.4 GB)

Additional details

Identifiers

DOI
10.22002/hc0gr-sm911
TILT_SERIES_ID
cwl2011-12-15-1

Funding

NIH
HHMI
Beckman Institute
Gordon and Betty Moore Foundation
Agouron Institute
John Templeton Foundation

Dates

Collected
2011-12-15
Created
2015-04-17
Updated
2015-04-17