Myxococcus xanthus PilO-sfGFP
Contributors
Researcher:
Description
Technical info
Tilt Series Date: 2014-03-07
Data Taken By: Yi-Wei Chang
Species / Specimen: Myxococcus xanthus
Strain:
Tilt Series Settings: Single Axis, tilt range: (-65.0°, 65.0°), step: 1.0°, constant angular increment, dosage: 200.0 eV/Ų, defocus: -8.0 μm, magnification: 26000x.
Microscope: Janelia Titan
Acquisition Software: UCSFTomo
Upload Method: pipeline
Processing Software Used: Raptor
Collaborators and Roles: Strain form Lotte Sogaard-Andersen
Purification / Growth Conditions / Treatment: Cells grown 48 hr in shaking CTT medium at 30 degrees without antibiotics. OD600=0.8
Sample Preparation: Vitrobot settings: 100% humidity, 3µl sample, 10 mg/ml BSA treated gold. -3.5 offset, 4 sec blot time, no drain. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200.
ycw2014-03-07-8_slicer8220.jpg: Filment bundle in Myxococcus Xanthus ycw2014-03-07-8_slicer8224.jpg: Filaments in Myxococcus Xanthus
Attached Files
20140306_YWC092_10007.mrc, Tilt Series (Pixel Size 4.2 nm), 3.6 GB
20140306_YWC092_10007_full.rec, Reconstruction (Pixel Size 16.8 nm), 688.9 MB
Files
keyimg_ycw2014-03-07-8.jpg
Files
(4.3 GB)
| Name | Size | |
|---|---|---|
| 3.6 GB | (external) Download | |
| 688.9 MB | (external) Download | |
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md5:c9371a19ca059f3cc492a363ee9f8a6d
|
1.0 MB | Preview Download |
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md5:307fbc5c2955e2c1aa85cfaf61c22c0c
|
25.0 MB | Preview Download |
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md5:0c0cc5ca6210d667726be6c454f41152
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388.7 kB | Preview Download |
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md5:50b13532cb120377acc3605c0cb397f7
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264.7 kB | Preview Download |
Additional details
Identifiers
- DOI
- 10.22002/hm1qd-59z19
- TILT_SERIES_ID
- ycw2014-03-07-8
Funding
- NIH
- HHMI
- Beckman Institute
- Gordon and Betty Moore Foundation
- Agouron Institute
- John Templeton Foundation
Dates
- Collected
-
2014-03-07
- Created
-
2014-03-08
- Updated
-
2014-07-24