E.cloacae-typeV-CdiA+
Contributors
Researchers:
Description
Technical info
Tilt Series Date: 2014-03-03
Data Taken By: Poorna Subramanian
Species / Specimen: Escherichia coli
Strain:
Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 190.0 eV/Ų, defocus: -10.0 μm, magnification: 22500x.
Microscope: Caltech Polara
Acquisition Software: Undefined
Upload Method: pipeline
Processing Software Used: Raptor
Collaborators and Roles: Drs. Davod Low and Chris Hayes, UC Santa Barbara
Purification / Growth Conditions / Treatment: Grew 2 ml LB culture from stock with Amp 150, Cm 100 till OD ~0.55, induced with 1.5mM IPTG and grown for 4h, then freeze as such, no spinning
Sample Preparation: Blot time 3s, Blot off-set -4, 3 ul culture
Attached Files
atlas10008.mrc, Tilt Series (Pixel Size 0.48 nm), 3.4 GB
atlas10008_part121_2.rec, Reconstruction (Pixel Size 1.92 nm), 801.8 MB
Files
keyimg_psa2014-03-03-8.jpg
Additional details
Identifiers
- DOI
- 10.22002/hnqyc-gkp67
- TILT_SERIES_ID
- psa2014-03-03-8
Funding
- NIH
- HHMI
- Beckman Institute
- Gordon and Betty Moore Foundation
- Agouron Institute
- John Templeton Foundation
Dates
- Collected
-
2014-03-03
- Created
-
2014-03-03
- Updated
-
2017-01-12