H.pylori 26695 WT
Contributors
Researcher:
Description
Technical info
Tilt Series Date: 2014-03-01
Data Taken By: Yi-Wei Chang
Species / Specimen: Helicobacter pylori
Strain: 26695
Tilt Series Settings: Single Axis, tilt range: (-65.0°, 65.0°), step: 1.0°, constant angular increment, dosage: 190.0 eV/Ų, defocus: -10.0 μm, magnification: 22500x.
Microscope: Caltech Polara
Acquisition Software: UCSFTomo
Upload Method: pipeline
Processing Software Used: Raptor
Collaborators and Roles: H.pylori cells from Carrie Shaffer
Purification / Growth Conditions / Treatment: H.pylori cells grown in BLBB + 5% heat inactivated fetal bovine serum at 37 degrees in 5% CO2 chamber to OD600=1, back diluted to OD 0.3, grew to OD 0.6 before frozen.
Sample Preparation: Vitrobot settings: 100% humidity, 3µl sample, 10 mg/ml BSA treated gold. -3.5 offset, 4 sec blot time, no drain. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200.
ycw2014-03-01-18_slicer9157.jpg: Helicobacter pylori . Peptidoglycan ycw2014-03-01-18_slicer9158.jpg: Helicobacter pylori . filament
Attached Files
20140301_YWC089_20010.mrc, Tilt Series (Pixel Size 0.48 nm), 3.7 GB
20140301_YWC089_20010_part131_3.rec, Reconstruction (Pixel Size 1.92 nm), 712.7 MB
Files
keyimg_ycw2014-03-01-18.jpg
Files
(4.5 GB)
| Name | Size | |
|---|---|---|
| 3.7 GB | (external) Download | |
| 712.7 MB | (external) Download | |
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md5:2f053c656fe063c4b0d9121bd8d0b1e2
|
1.1 MB | Preview Download |
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md5:1be6f47d7307020da4da376c251f8242
|
27.5 MB | Preview Download |
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md5:fcdd0a5317e15aff0c4257aefd4d92b1
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346.6 kB | Preview Download |
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md5:3b7199954a38483d5f551495705fdc0c
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418.6 kB | Preview Download |
Additional details
Identifiers
- DOI
- 10.22002/hqfpp-nxc26
- TILT_SERIES_ID
- ycw2014-03-01-18
Funding
- NIH
- HHMI
- Beckman Institute
- Gordon and Betty Moore Foundation
- Agouron Institute
- John Templeton Foundation
Dates
- Collected
-
2014-03-01
- Created
-
2014-03-02
- Updated
-
2014-08-01