Published 2015 | Version v1
Dataset Open

COS7pagfp-CHMP4a cells

  • 1. ROR icon California Institute of Technology

Description

Cos7 PAGFP CHMP4A cells synchronized with Thymidine 2mM.Looking for midbodies or tunneling nanotubes

Technical info

Tilt Series Date: 2015-03-08

Data Taken By: Rasika Ramdasi

Species / Specimen: Cos7

Strain: Cos7 cells

Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 180.0 eV/Ų, defocus: -10.0 μm, magnification: 19500x.

Microscope: Caltech Polara

Acquisition Software: UCSFTomo

Upload Method: pipeline

Processing Software Used: UCSF tomo

Sample Preparation: synchronized with Thymidine 2mM., freeze after 14 hours of thymidine treatment.

Attached Files

Cos7-2-atlasB0034.mrc, Tilt Series (Pixel Size 0.56 nm), 3.3 GB

Cos7-2-atlasB0034_part121_12.rec, Reconstruction (Pixel Size 2.24 nm), 602.8 MB

Files

keyimg_rra2015-03-08-34.jpg

Files (4.0 GB)

Name Size
3.3 GB (external) Download
602.8 MB (external) Download
md5:d43a1f6aaec7018e2273dd4f2d0b259d
1.1 MB Preview Download
md5:24b858fbdba03540a0c947c46922ee42
22.2 MB Preview Download

Additional details

Identifiers

DOI
10.22002/hwyfx-6y652
TILT_SERIES_ID
rra2015-03-08-34

Funding

NIH
HHMI
Beckman Institute
Gordon and Betty Moore Foundation
Agouron Institute
John Templeton Foundation

Dates

Collected
2015-03-08
Created
2015-03-09
Updated
2015-03-26