Caltech Tomography Archive
Published 2019 | Version v1
Dataset Open

r-cem1-1_ts6

Nicolas, William1
  • 1. ROR icon California Institute of Technology
Researcher:
Ladinsky, Mark

Description

R-CEM1-1 - Root tips

Files

keyimg_wnj2019-02-22-4.jpg
Files (8.6 MB)
Name Size
keymov_wnj2019-02-22-4.mp4
md5:7f8a2b2fce257bd42e960e3e898ac8c2
7.5 MB Download
keyimg_wnj2019-02-22-4.jpg
md5:76682f703b7660595f97653bc4454c07
1.1 MB Preview Download

Technical info

Tilt Series Date: 2019-02-22

Data Taken By: William Nicolas

Species / Specimen: Arabidopsis thaliana

Strain: Col-0

Tilt Series Settings: Single Axis, tilt range: (-60.0°, 50.0°), step: °, constant angular increment, dosage: 82.0 eV/Ų, defocus: -10.0 μm, magnification: 33000x.

Microscope: Caltech Titan Krios

Acquisition Software: Serial EM

Upload Method: webload

Processing Software Used: IMOD

Collaborators and Roles: Mark LadinskyAmelie Leforestier

Purification / Growth Conditions / Treatment: 1 month old lateral root tips were sampled and frozen in gold platted copper freezer hats (cavity 0.1mm) with 25% Dextran. Freezing was done at Gif-Sur-Yvette with Amelie Leforestier

Sample Preparation: Cryosections (150nm) collected by Mark Ladinsky.

External Files

Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/wnj2019-02-22-4/rawdata/

r-cem1-1_ts6_orig.st, Tilt Series (Pixel Size 0.43 nm), 1.6 GB Download

r-cem1-1_ts6.rec, Reconstruction (Pixel Size 1.296 nm), 1.0 GB Download

Additional details

Identifiers Funding Dates
Created:
June 6, 2023
Modified:
August 29, 2025