Hyphomonas AR46
Contributors
Researcher:
Description
Technical info
Tilt Series Date: 2012-09-07
Data Taken By: Yi-Wei Chang
Species / Specimen: Hyphomonas neptunium
Strain: ATCC 15444
Tilt Series Settings: Single Axis, tilt range: (-65.0°, 65.0°), step: 1.0°, constant angular increment, dosage: 100.0 eV/Ų, defocus: -10.0 μm, magnification: 22500x.
Acquisition Software: UCSFTomo
Upload Method: pipeline
Processing Software Used: Raptor
Collaborators and Roles: Strain from Martin Thanbichler
Purification / Growth Conditions / Treatment: Cells grown overnight in Marine Broth (MB) medium at 30 degrees without antibiotics. OD600=0.6
Sample Preparation: Vitrobot settings: 100% humidity, 3µl sample, 10 nm BSA treated gold. -3 offset, 4 sec blot time, no drain. Ethane/propane mix.
ycw2012-09-07-19_slicer7052.jpg: REZ ycw2012-09-07-19_slicer7057.jpg: Filament
Attached Files
20120907_AR46_10019.mrc, Tilt Series (Pixel Size 1.0 nm), 925.0 MB
20120907_AR46_10019_part132_2.rec, Reconstruction (Pixel Size 2.0 nm), 262.8 MB
Files
keyimg_ycw2012-09-07-19.jpg
Files
(1.2 GB)
| Name | Size | |
|---|---|---|
| 925.0 MB | (external) Download | |
| 262.8 MB | (external) Download | |
|
md5:c0868c717246a4cbe3d50d873a4fe162
|
991.0 kB | Preview Download |
|
md5:23ffc54c51b5cdffb92681acc514223f
|
9.5 MB | Preview Download |
|
md5:e8e25514b22e33172d2c6653396e19df
|
350.9 kB | Preview Download |
|
md5:fa5dec30f69b3e9a533c88ab7f81ebbd
|
310.2 kB | Preview Download |
Additional details
Identifiers
- DOI
- 10.22002/j1chp-djb08
- TILT_SERIES_ID
- ycw2012-09-07-19
Funding
- NIH
- HHMI
- Beckman Institute
- Gordon and Betty Moore Foundation
- Agouron Institute
- John Templeton Foundation
Dates
- Collected
-
2012-09-07
- Created
-
2012-09-08
- Updated
-
2014-07-16