Myxococcus xanthus LL061
Contributors
Researcher:
Description
Technical info
Tilt Series Date: 2013-04-24
Data Taken By: Yi-Wei Chang
Species / Specimen: Myxococcus xanthus
Strain:
Tilt Series Settings: Single Axis, tilt range: (-65.0°, 65.0°), step: 1°, constant angular increment, dosage: 200.0 eV/Ų, defocus: -10.0 μm, magnification: 29100x.
Acquisition Software: FEIbatchtomo
Upload Method: pipeline
Processing Software Used: Raptor
Collaborators and Roles: Strain from Martin Thanbichler
Purification / Growth Conditions / Treatment: Cells grown 48 hr in CTT medium at 30 degrees without antibiotics. OD600=0.7
Sample Preparation: Vitrobot settings: 100% humidity, 3µl sample, 10 mg/ml BSA treated gold. -3 offset, 4 sec blot time, no drain. Ethane/propane mix. Grid: Quantifoil Au Finder 2/2.
Attached Files
LL061_UCLA_correlated_20130424_13.mrc, Tilt Series (Pixel Size 1.0 nm), 1.1 GB
LL061_UCLA_correlated_20130424_13_full.rec, Reconstruction (Pixel Size 2.0 nm), 629.1 MB
LL061_UCLA_correlated_20130424_13_bin2_DB.rec, Reconstruction (Pixel Size 0.0 nm), 314.6 MB
Files
keyimg_ycw2013-04-24-11.jpg
Additional details
Identifiers
- DOI
- 10.22002/j8a6v-87d79
- TILT_SERIES_ID
- ycw2013-04-24-11
Funding
- NIH
- HHMI
- Beckman Institute
- Gordon and Betty Moore Foundation
- Agouron Institute
- John Templeton Foundation
Dates
- Collected
-
2013-04-24
- Created
-
2013-04-27
- Updated
-
2013-05-24