Myxococcus xanthus pilM-YFP
Contributors
Researcher:
Description
Technical info
Tilt Series Date: 2014-05-16
Data Taken By: Yi-Wei Chang
Species / Specimen: Myxococcus xanthus
Strain:
Tilt Series Settings: Single Axis, tilt range: (-65.0°, 65.0°), step: 1.0°, constant angular increment, dosage: 200.0 eV/Ų, defocus: -8.0 μm, magnification: 26000x.
Microscope: Janelia Titan
Acquisition Software: UCSFTomo
Upload Method: pipeline
Processing Software Used: Raptor
Collaborators and Roles: Strain form Lotte Sogaard-Andersen
Purification / Growth Conditions / Treatment: Cells grown 48 hr in shaking CTT medium at 30 degrees without antibiotics. OD600=0.9
Sample Preparation: Vitrobot settings: 100% humidity, 3µl sample, 10 mg/ml BSA treated 10 nm gold. -4 offset, 4 sec blot time, no drain. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200.
ycw2014-05-16-23_slicer6220.jpg: secretion system? . unknown tube? ycw2014-05-16-23_slicer6221.jpg: unknown
Attached Files
20140516_Janelia_YWC096_10045_full.rec, Reconstruction (Pixel Size 1.32 nm), 688.9 MB
20140516_Janelia_YWC096_10045.mrc, Tilt Series (Pixel Size 0.33 nm), 3.6 GB
Files
keyimg_ycw2014-05-16-23.jpg
Files
(4.3 GB)
| Name | Size | |
|---|---|---|
| 3.6 GB | (external) Download | |
| 688.9 MB | (external) Download | |
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md5:3941a530db390abe62d14e5abf74f5b4
|
987.5 kB | Preview Download |
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md5:1d0eee166aba10af94ebce8c732f6295
|
22.8 MB | Preview Download |
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md5:d48abf3a0731c43807f814fa192edc04
|
320.7 kB | Preview Download |
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md5:f222c57778afb7d757b06bbc2f1972f8
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403.7 kB | Preview Download |
Additional details
Identifiers
- DOI
- 10.22002/jaqj4-a9j07
- TILT_SERIES_ID
- ycw2014-05-16-23
Funding
- NIH
- HHMI
- Beckman Institute
- Gordon and Betty Moore Foundation
- Agouron Institute
- John Templeton Foundation
Dates
- Collected
-
2014-05-16
- Created
-
2014-05-19
- Updated
-
2014-07-11