gh9-3_sq3_cell3
Description
Technical info
Tilt Series Date: 2018-10-10
Data Taken By: William Nicolas
Species / Specimen: Gluconacetobacter hansenii
Strain: ATCC 23769
Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 2°, constant angular increment, dosage: 130.0 eV/Ų, defocus: -8.0 μm, magnification: 22500x.
Microscope: Caltech Polara
Acquisition Software: Undefined
Upload Method: pipeline
Processing Software Used: RAPTOR
Purification / Growth Conditions / Treatment: Overnight 6mL culture - Shaken, centrif 2500rcf 10min 30C, resuspended in 0.5mL SH medium.
Sample Preparation: +13min post-prep' - 30C, 100% hum Manual backblot 5s FM layed on grid prior and backblotted to dry excess liquid.
Attached Files
gh9-3_sq3_cell3.mrc, Tilt Series (Pixel Size 0.48 nm), 1.7 GB
gh9-3_sq3_cell3_full.rec, Reconstruction (Pixel Size 0.96 nm), 2.8 GB
Files
keyimg_wnj2018-10-10-5.jpg
Additional details
Identifiers
- DOI
- 10.22002/jbpqc-zdj47
- TILT_SERIES_ID
- wnj2018-10-10-5
Funding
- NIH
- HHMI
- Beckman Institute
- Gordon and Betty Moore Foundation
- Agouron Institute
- John Templeton Foundation
Dates
- Collected
-
2018-10-10
- Created
-
2018-10-11
- Updated
-
2018-10-12