E.coli "strain 2" P221D
Contributors
Researcher:
Description
Technical info
Tilt Series Date: 2012-03-08
Data Taken By: Ariane Briegel
Species / Specimen: Escherichia coli
Strain: RP 437
Tilt Series Settings: Single Axis, tilt range: (-63.0°, 63.0°), step: 1°, constant angular increment, dosage: 160.0 eV/Ų, defocus: -10.0 μm, magnification: 34000x.
Acquisition Software: UCSFTomo
Upload Method: pipeline
Processing Software Used: Raptor
Collaborators and Roles: John S. PArkinson
Purification / Growth Conditions / Treatment: cells grown overnight in TB medium with gentamycin at 30 degrees. Diluted in fresh medium and grown to log phase, then incubated with penicillin for one hour.
Sample Preparation: Vitrobot settings: -4 offset, 3.5 s blot time, no drain, 100 percent humidity
Attached Files
strain2marcheightgrid2atlas20002.mrc, Tilt Series (Pixel Size 0.644 nm), 1.1 GB
strain2marcheightgrid2atlas20002_part128_7.rec, Reconstruction (Pixel Size 1.288 nm), 516.1 MB
Files
keyimg_aba2012-03-08-4.jpg
Additional details
Identifiers
- DOI
- 10.22002/je27q-05p82
- TILT_SERIES_ID
- aba2012-03-08-4
Funding
- NIH
- HHMI
- Beckman Institute
- Gordon and Betty Moore Foundation
- Agouron Institute
- John Templeton Foundation
Dates
- Collected
-
2012-03-08
- Created
-
2012-03-08
- Updated
-
2012-03-14