Published 2016 | Version v1
Dataset Open

INS-1E with Calreticulin-YFP

  • 1. ROR icon California Institute of Technology

Description

CLEM and ECT

Technical info

Tilt Series Date: 2016-04-17

Data Taken By: Shrawan kumar Mageswaran

Species / Specimen: INS-1E Pancreatic Cells expressing Calreticulin-YFP

Strain:

Tilt Series Settings: Single Axis, tilt range: (-55.0°, 55.0°), step: 1°, constant angular increment, dosage: 0.47368421 eV/Ų, defocus: -8.0 μm, magnification: 18000x.

Microscope: Caltech Polara

Acquisition Software: SerialEM

Upload Method: pipeline

Processing Software Used: IMOD

Collaborators and Roles: Zachary Freyberg from University of Pittsburgh provided the frozen cells on grids. Collaboration via Stephen Carter. CLEM and ECT done by me.

Attached Files

Calreticulin-TS7.st, Tilt Series (Pixel Size 0.6 nm), 3.1 GB

Calreticulin-TS7_full.rec, Reconstruction (Pixel Size 2.4 nm), 1.1 GB

Files

keyimg_smk2016-04-17-28.jpg

Files (4.2 GB)

Name Size
3.1 GB (external) Download
1.1 GB (external) Download
md5:a703f011e5a599c22199139f2b2e902d
1.1 MB Preview Download
md5:573b53144a15d7d6c888936b6db80440
27.9 MB Preview Download

Additional details

Identifiers

DOI
10.22002/jghe1-hrs87
TILT_SERIES_ID
smk2016-04-17-28

Funding

NIH
HHMI
Beckman Institute
Gordon and Betty Moore Foundation
Agouron Institute
John Templeton Foundation

Dates

Collected
2016-04-17
Created
2016-04-22
Updated
2019-04-19