Published 2011 | Version v1
Dataset Open

COS7 CHMP4A+VPS4DN

  • 1. ROR icon California Institute of Technology

Contributors

Description

Cos7 cells transfected with CHMP4A & VPS4DN constructs

Technical info

Tilt Series Date: 2011-12-22

Data Taken By: Cora Woodward

Species / Specimen: Cercopithecus aethiops (monkey, African green)

Strain: COS-7 ATCC CRL-1651

Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 150.0 eV/Ų, defocus: -6.0 μm, magnification: 22500x.

Acquisition Software: UCSF tomo

Upload Method: rundir

Processing Software Used: imod

Collaborators and Roles: Constructs provided by Wes Sundquist. Cells transfected, frozen and imaged at Caltech by Cora Woodward

Purification / Growth Conditions / Treatment: whole cells grown on grids and transfected with lipofectamine

Sample Preparation: manual blotting

Attached Files

11dec22_C104_1_AT3BT1_0009.st, Tilt Series (Pixel Size 0.5 nm), 1.0 GB

11dec22_C104_1_AT3BT1_0009_full.rec, Reconstruction (Pixel Size 2.0 nm), 953.8 MB

11dec22_C104_1_AT3BT1_0009_full.tiff, file associated to 3D Image #68121, 975.5 kB

Files

11dec22_C104_1_AT3BT1_0009_full.tiff

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Files (2.0 GB)

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1.0 GB (external) Download
953.8 MB (external) Download
975.5 kB (external) Download
md5:c593c4e9628593d3aa4b2acebb0c9560
1.1 MB Preview Download
md5:418ada3c29a5185a0c3da5f0001dcadd
17.5 MB Preview Download

Additional details

Identifiers

DOI
10.22002/jh201-mxm72
TILT_SERIES_ID
cwl2011-12-22-11

Funding

NIH
HHMI
Beckman Institute
Gordon and Betty Moore Foundation
Agouron Institute
John Templeton Foundation

Dates

Collected
2011-12-22
Created
2015-09-16
Updated
2015-09-16