Published 2015 | Version v1
Dataset Open

COS7pagfp-CHMP4a cells

  • 1. ROR icon California Institute of Technology

Description

Cos7 PAGFP CHMP4A cells synchronized with Thymidine 2mM.Looking for midbodies or tunneling nanotubes

Technical info

Tilt Series Date: 2015-03-25

Data Taken By: Rasika Ramdasi

Species / Specimen: Cos7

Strain: Cos7 cells

Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 180.0 eV/Ų, defocus: -8.0 μm, magnification: 22500x.

Microscope: Caltech Polara

Acquisition Software: UCSFTomo

Upload Method: pipeline

Processing Software Used: UCSF tomo

Sample Preparation: synchronized with Thymidine 2mM., freeze after 14 hours of thymidine treatment.

Attached Files

cos7pagfp60010.mrc, Tilt Series (Pixel Size 0.48 nm), 3.4 GB

cos7pagfp60010_part121_26.rec, Reconstruction (Pixel Size 1.92 nm), 623.6 MB

Files

keyimg_rra2015-03-25-13.jpg

Files (4.1 GB)

Name Size
3.4 GB (external) Download
623.6 MB (external) Download
md5:7aae11e8d8d6884fde89bd99fc21f2ba
1.1 MB Preview Download
md5:e5a477bf2eeb57c2eb4eef77eefa9190
20.1 MB Preview Download

Additional details

Identifiers

DOI
10.22002/jk73p-n9d47
TILT_SERIES_ID
rra2015-03-25-13

Funding

NIH
HHMI
Beckman Institute
Gordon and Betty Moore Foundation
Agouron Institute
John Templeton Foundation

Dates

Collected
2015-03-25
Created
2015-03-26
Updated
2015-03-26