perfusion_dcrp_fixed
Contributors
Researcher:
Description
Technical info
Tilt Series Date: 2015-08-18
Data Taken By: Poorna Subramanian
Species / Specimen: Shewanella oneidensis
Strain: MR-1
Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 150.0 eV/Ų, defocus: -10.0 μm, magnification: 18000x.
Microscope: Caltech Polara
Acquisition Software: UCSFTomo
Upload Method: pipeline
Processing Software Used: Batchruntomo
Collaborators and Roles: Moh and Sahand
Purification / Growth Conditions / Treatment: The sample was grown in perfusion chamber and fixed for 1hour with 2.5% glutaraldehyde. Then washed 3 times in distilled water for 1 minute each. The sample was blotted in a manual plunger with 1ul gold beads.
Sample Preparation: The ice was too thick in the squares for which LM images were available, so could not correlate. Took tomograms of all bugs in a square.
Attached Files
perfusion_dcrp_atlas10024.rec, Reconstruction (Pixel Size 2.4 nm), 889.0 MB
perfusion_dcrp_atlas10024.mrc, Tilt Series (Pixel Size 0.6 nm), 3.4 GB
Files
keyimg_psa2015-08-18-24.jpg
Additional details
Identifiers
- DOI
- 10.22002/jnak3-vdt68
- TILT_SERIES_ID
- psa2015-08-18-24
Funding
- NIH
- HHMI
- Beckman Institute
- Gordon and Betty Moore Foundation
- Agouron Institute
- John Templeton Foundation
Dates
- Collected
-
2015-08-18
- Created
-
2015-08-19
- Updated
-
2015-08-28