FtsZ on both sides

Description

Technical info

Tilt Series Date: 2015-03-10

Data Taken By: Qing Yao

Species / Specimen: Caulobacter crescentus

Strain: NA1000

Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 180.0 eV/Ų, defocus: -10.0 μm, magnification: 27500x.

Microscope: Caltech Polara

Acquisition Software: UCSF Tomo

Upload Method: pipeline

Processing Software Used: Raptor

Purification / Growth Conditions / Treatment: C. crescentus was from Yi-Wei's cryo-stock and was grown in PYE medium overnight at 30°c 250 rpm. In the next days' morning, 1/20 subculture into the M2G medium. When OD600 reaches 0.30, 2 ml medium was spun down gently (3000 rpm for 3 min) and the resuspension was 100 ul for cryo-grid preparation.

Sample Preparation: Glow discharged Quantifoil R2/R holey carbon film. 4 ul concentrated gold suspension was added to 16 ul bacteria and mix before plunge freezing. Plunge freezing condition: 6(force)-2(duration time)-1(dry time). Tilt Series Setting: single tilt. constant angular increment, step: 1. tilt range: (-60, 60). dosage: 180/A2. defocus: -10 um. magnification: 27500. Tilt Series Setting: single tilt. constant angular increment, step: 1.0. tilt range: (-60, 60).

qya2015-03-10-16_slicer12914.jpg: Invagination qya2015-03-10-16_slicer12915.jpg: FtsZ ring on both side, FstZ ring the left appears incomplete and ends before the missing wedge.

Attached Files

Qing15_10016.mrc, Tilt Series (Pixel Size 0.39 nm), 3.4 GB

Qing15_10016_full.rec, Reconstruction (Pixel Size 1.56 nm), 445.4 MB