Published 2007 | Version v1
Dataset Open

XL-10 Sacculus

Creators

  • 1. ROR icon California Institute of Technology

Description

E. coli XL-10 Gold sacculus .

Technical info

Tilt Series Date: 2007-06-27

Data Taken By: Lu Gan

Species / Specimen: Escherichia coli

Strain: XL10-Gold

Tilt Series Settings: Single Axis, tilt range: (-70.0°, 70.0°), step: 2.0°, constant angular increment, dosage: 100.0 eV/Ų, defocus: -6.0 μm, magnification: 0x.

Acquisition Software: UCSF Tomo

Upload Method: rundir

Processing Software Used: imod

Collaborators and Roles: prepared by Lu Gan at Caltech; data collected by Lu Gan; processed by Lu Gan

Purification / Growth Conditions / Treatment: Grown to exponential phase in LB; Purified by hot SDS

Sample Preparation: C-flat 4/2, BSA treated 5nm gold, 100% humidity, -2mm offset,

Attached Files

EC_05.mrc, Tilt Series (Pixel Size 0.78 nm), 595.7 MB

EC_05.rec, Reconstruction (Pixel Size 0.78 nm), 701.3 MB

Files

keyimg_lga2007-06-27-2.jpg

Files (1.3 GB)

Name Size
595.7 MB (external) Download
701.3 MB (external) Download
md5:8f5fb090a40332e11b8c52d902a8136c
3.1 MB Preview Download
md5:7a0e23f386aca94d5c2788a2c2f61585
2.8 MB Preview Download

Additional details

Identifiers

DOI
10.22002/jrvys-t8516
TILT_SERIES_ID
lga2007-06-27-2

Funding

NIH
HHMI
Beckman Institute
Gordon and Betty Moore Foundation
Agouron Institute
John Templeton Foundation

Dates

Collected
2007-06-27
Created
2008-08-01
Updated
2022-11-03