Published 2016 | Version v1
Dataset Open

Dividing Caulobacter

Creators

  • 1. ROR icon California Institute of Technology

Description

Dividing Caulobacter, low-quality tomograms

Technical info

Tilt Series Date: 2016-02-28

Data Taken By: Qing Yao

Species / Specimen: Caulobacter crescentus

Strain: NA1000

Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 180.0 eV/Ų, defocus: -11.0 μm, magnification: 22500x.

Microscope: Caltech Polara

Acquisition Software: UCSFTomo

Upload Method: pipeline

Processing Software Used: Raptor

Purification / Growth Conditions / Treatment: Glow discharged Quantifoil R2/R holey carbon film. 4 ul concentrated gold suspension was added to 16 ul bacteria and mix before plunge freezing. Plunge freezing condition: 6(force)-2(duration time)-1(dry time). Tilt Series Setting: single tilt. constant angular increment, step: 1. tilt range: (-60, 60). dosage: 180/A2. defocus: -10 um. magnification: 27500.

Sample Preparation: single tilt. constant angular increment, step: 1.0. tilt range: (-60, 60). dosage: 180/A2. defocus: -10um. magnification: 27500.

Attached Files

view80010.mrc, Tilt Series (Pixel Size 0.48 nm), 3.4 GB

view80010_part121_60.rec, Reconstruction (Pixel Size 1.92 nm), 445.4 MB

Files

keyimg_qya2016-02-28-10.jpg

Files (3.9 GB)

Name Size
md5:74cf6a81f7b63deb65daa7a658ba1e1f
865.5 kB Preview Download
md5:20c1eceecdb980ca064a176dd4e40ff1
12.0 MB Preview Download
3.4 GB (external) Download
445.4 MB (external) Download

Additional details

Identifiers

DOI
10.22002/jwqf6-kdn28
TILT_SERIES_ID
qya2016-02-28-10

Funding

NIH
HHMI
Beckman Institute
Gordon and Betty Moore Foundation
Agouron Institute
John Templeton Foundation

Dates

Collected
2016-02-28
Created
2016-02-29
Updated
2022-10-12