Caltech Tomography Archive
Published 2018 | Version v1
Dataset Open

Shewanella OMV delMTR

Metskas, LaurenAnn1
  • 1. ROR icon California Institute of Technology
Researchers:
Zacharoff, Lori
Metskas, Lauren Ann

Description

Shewanella outer membrane vesicles, delMTR (cytochromes) knockout, high resolution

Files

keyimg_lma2018-09-12-20.jpg
Files (10.7 MB)
Name Size
keyimg_lma2018-09-12-20.jpg
md5:75836fed8466cf79d727fb80837ea92e
1.0 MB Preview Download
keymov_lma2018-09-12-20.mp4
md5:b83e6c8aa16ebe92d9d8642c0f7e14ba
9.6 MB Download

Technical info

Tilt Series Date: 2018-09-12

Data Taken By: LaurenAnn Metskas

Species / Specimen: Shewanella oneidensis

Strain: MR-1

Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 3°, constant angular increment, dosage: 120.0 eV/Ų, defocus: 0.0 μm, magnification: 82000x.

Microscope: None

Acquisition Software: Serial EM

Upload Method: rundir

Processing Software Used: IMOD, ctffind4, custom scripts

Collaborators and Roles: purified by Lori Zacharoff in the El Naggar lab at USC; data collected by Lauren Ann Metskas; data processed by Lauren Ann Metskas

Sample Preparation: C-Flat 2/2-300 mesh grid, 10nm BSA-gold, 100% humidity, -1.5 to -5 micron defocus

External Files

Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/lma2018-09-12-20/rawdata/

37_dose-filt.st, Tilt Series (Pixel Size 0.1778 nm), 2.3 GB Download

37_dose-filt.rec, Reconstruction (Pixel Size 0.7112 nm), 1.0 GB Download

TS_37.mrc.mdoc, 24.9 kB Download

frames.tar.bz2, zipped raw frames, 2.5 GB Download

scripts.tar.bz2, zipped processing scripts, 7.9 kB Download

Additional details

Identifiers Funding Dates
Created:
June 4, 2023
Modified:
August 23, 2025