LCV_Legionella pneumophila
Contributors
Researchers:
Description
Technical info
Tilt Series Date: 2017-06-01
Data Taken By: Debnath Ghosal
Species / Specimen: Legionella pneumophila
Strain: Philadelphia-1_Lp02
Tilt Series Settings: Single Axis, tilt range: (-54.0°, 54.0°), step: 2°, constant angular increment, dosage: 110.0 eV/Ų, defocus: -7.0 μm, magnification: 27500x.
Microscope: Caltech Polara
Acquisition Software: UCSFTomo
Upload Method: pipeline
Processing Software Used: Raptor
Collaborators and Roles: Joelle Gabay and Howard Shuman prepared samples, Debnath Ghosal collected data.
Purification / Growth Conditions / Treatment: Phagosome isolation method as described by Joelle Gabay (Endnote).
Sample Preparation: 6 grids were frozen, cell density was increased to 2x by centrifugation. Vitrobot (MK iv) settings: 100% humidity, 4µl sample, Blot force 6, blot time 4.5 sec. Wait time 0 sec. Drain time 1 sec. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200. Add gold to cells prior to freezing: 1ul of gold to 4 ul of cells, freeze.
Attached Files
LCV_20170601p0002_part55_3.rec, Reconstruction (Pixel Size 1.56 nm), 445.4 MB
LCV_20170601p0002.mrc, Tilt Series (Pixel Size 0.39 nm), 1.6 GB
Files
keyimg_dga2017-06-01-29.jpg
Additional details
Identifiers
- DOI
- 10.22002/jwyqk-knn54
- TILT_SERIES_ID
- dga2017-06-01-29
Funding
- NIH
- HHMI
- Beckman Institute
- Gordon and Betty Moore Foundation
- Agouron Institute
- John Templeton Foundation
Dates
- Collected
-
2017-06-01
- Created
-
2017-06-05
- Updated
-
2017-07-05