COS7 VPS4DN+CHMP4A
Description
Technical info
Tilt Series Date: 2012-02-04
Data Taken By: Cora Woodward
Species / Specimen: Cercopithecus aethiops (monkey, African green)
Strain: COS-7 ATCC CRL-1651
Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 150.0 eV/Ų, defocus: -6.0 μm, magnification: 22500x.
Acquisition Software: UCSF tomo
Upload Method: rundir
Processing Software Used: imod
Collaborators and Roles: VPS4DN and CHMP4A constructs provided by Wes Sundquist. Cells transfected, frozen and imaged at Caltech by Cora Woodward
Purification / Growth Conditions / Treatment: whole cells grown on grids and transfected with lipofectamine
Sample Preparation: manual blotting
Attached Files
12feb04_ATBT1_0022_full.rec, Reconstruction (Pixel Size 2.0 nm), 722.6 MB
12feb04_ATBT1_0022.mrc, Tilt Series (Pixel Size 0.5 nm), 1.0 GB
12feb04_ATBT1_0022_full.tiff, file associated to 3D Image #68719, 1.2 MB
Files
12feb04_ATBT1_0022_full.tiff
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Additional details
Identifiers
- DOI
- 10.22002/jyjay-jjn92
- TILT_SERIES_ID
- cwl2012-02-04-20
Funding
- NIH
- HHMI
- Beckman Institute
- Gordon and Betty Moore Foundation
- Agouron Institute
- John Templeton Foundation
Dates
- Collected
-
2012-02-04
- Created
-
2015-09-16
- Updated
-
2015-09-16