NR1769pRP4 conj NR1769 phage MS2
Contributors
Researcher:
Description
Technical info
Tilt Series Date: 2014-11-26
Data Taken By: Elitza Tocheva
Species / Specimen: Escherichia coli
Strain: NR1769
Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 180.0 eV/Ų, defocus: -10.0 μm, magnification: 22500x.
Microscope: Caltech Polara
Acquisition Software: UCSFTomo
Upload Method: reupload_downloaded
Processing Software Used: IMOD
Collaborators and Roles: Christian Baron
Purification / Growth Conditions / Treatment: cells were grown to log phase and mixed in 1:1 ratio on 2% agar plate and left to conjugate for 30min. Phage MS2 was added to visualize any F pili
Sample Preparation: Vitrobot
Attached Files
5plus7_ms20009.mrc, Tilt Series (Pixel Size 0.5 nm), 3.4 GB
5plus7_ms20009.rec, Reconstruction (Pixel Size 0.0 nm), 711.2 MB
Files
keyimg_etl2014-11-26-9.jpg
Additional details
Identifiers
- DOI
- 10.22002/k2sqf-vbq36
- TILT_SERIES_ID
- etl2014-11-26-9
Funding
- NIH
- HHMI
- Beckman Institute
- Gordon and Betty Moore Foundation
- Agouron Institute
- John Templeton Foundation
Dates
- Collected
-
2014-11-26
- Created
-
2014-12-02
- Updated
-
2014-12-19