Published 2014 | Version v1
Dataset Open

Vibrio delta-CheA3

  • 1. ROR icon California Institute of Technology

Contributors

Researcher:

Description

Vibrio cholerae strain deleted for CheA3

Technical info

Tilt Series Date: 2014-03-05

Data Taken By: Ariane Briegel

Species / Specimen: Vibrio cholerae

Strain: O1 biovar El Tor

Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 160.0 eV/Ų, defocus: -10.0 μm, magnification: 33000x.

Microscope: Janelia Titan

Acquisition Software: UCSFTomo

Upload Method: pipeline

Processing Software Used: Raptor

Collaborators and Roles: Ikuro Kawagishi

Purification / Growth Conditions / Treatment: Grew in LB 24 hours at 30C; diluted 150 µL into 2 mL Ca-HEPES buffer and grew 16 hours at 30C

Attached Files

deltaA3VibrioC0010.mrc, Tilt Series (Pixel Size 0.33 nm), 3.3 GB

deltaA3VibrioC0010_full.rec, Reconstruction (Pixel Size 1.32 nm), 688.9 MB

Files

keyimg_aba2014-03-05-14.jpg

Files (4.1 GB)

Name Size
3.3 GB (external) Download
688.9 MB (external) Download
md5:840906b8f14157d3996bcaa668b95eaf
1.1 MB Preview Download
md5:f79f51d4755a17e85641f48ec5cc5cb5
29.3 MB Preview Download

Additional details

Identifiers

DOI
10.22002/k33ce-nbk60
TILT_SERIES_ID
aba2014-03-05-14

Funding

NIH
HHMI
Beckman Institute
Gordon and Betty Moore Foundation
Agouron Institute
John Templeton Foundation

Dates

Collected
2014-03-05
Created
2014-03-07
Updated
2014-09-24