Myxococcus xanthus PilO-sfGFP
Contributors
Researcher:
Description
Technical info
Tilt Series Date: 2014-04-15
Data Taken By: Yi-Wei Chang
Species / Specimen: Myxococcus xanthus
Strain:
Tilt Series Settings: Single Axis, tilt range: (-65.0°, 65.0°), step: 1.0°, constant angular increment, dosage: 200.0 eV/Ų, defocus: -8.0 μm, magnification: 27500x.
Microscope: Caltech Polara
Acquisition Software: UCSFTomo
Upload Method: pipeline
Processing Software Used: Raptor
Collaborators and Roles: Strain form Lotte Sogaard-Andersen
Purification / Growth Conditions / Treatment: Cells grown 48 hr in shaking CTT medium at 30 degrees without antibiotics. OD600=0.8
Sample Preparation: Vitrobot settings: 100% humidity, 3µl sample, 10 mg/ml BSA treated gold. -3.5 offset, 4 sec blot time, no drain. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200.
ycw2014-04-15-12_slicer7452.jpg: filament bundle . T6SS? . in Myxococcus xanthus PilO-sfGFP ycw2014-04-15-12_slicer7453.jpg: filaments in Myxococcus xanthus PilO-sfGFP
Attached Files
20140415_YWC092_10012.mrc, Tilt Series (Pixel Size 0.39 nm), 3.7 GB
20140415_YWC092_10012_full.rec, Reconstruction (Pixel Size 1.56 nm), 712.7 MB
Files
keyimg_ycw2014-04-15-12.jpg
Files
(4.5 GB)
| Name | Size | |
|---|---|---|
| 3.7 GB | (external) Download | |
| 712.7 MB | (external) Download | |
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md5:8d747c8f983732751aa326a9d1f750a5
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1.0 MB | Preview Download |
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md5:58881b049d839e0220bcaf4d725c6165
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24.2 MB | Preview Download |
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md5:df9010fa6e1801a94beb61d7ce1cbea9
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330.2 kB | Preview Download |
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md5:8a2f1c0d1627cafcf02efaea7bb215fd
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328.7 kB | Preview Download |
Additional details
Identifiers
- DOI
- 10.22002/k7rt7-pkh79
- TILT_SERIES_ID
- ycw2014-04-15-12
Funding
- NIH
- HHMI
- Beckman Institute
- Gordon and Betty Moore Foundation
- Agouron Institute
- John Templeton Foundation
Dates
- Collected
-
2014-04-15
- Created
-
2014-04-15
- Updated
-
2014-07-18