Myxococcus xanthus delta-pilT
Contributors
Researcher:
Description
Technical info
Tilt Series Date: 2013-12-21
Data Taken By: Yi-Wei Chang
Species / Specimen: Myxococcus xanthus
Strain:
Tilt Series Settings: Single Axis, tilt range: (-65.0°, 65.0°), step: 1.0°, constant angular increment, dosage: 180.0 eV/Ų, defocus: -6.0 μm, magnification: 27500x.
Microscope: Caltech Polara
Acquisition Software: UCSFTomo
Upload Method: pipeline
Processing Software Used: Raptor
Collaborators and Roles: Strain form Lotte Sogaard-Andersen
Purification / Growth Conditions / Treatment: Cells grown 48 hr in shaking CTT medium at 30 degrees without antibiotics. OD600=0.7
Sample Preparation: Vitrobot settings: 100% humidity, 3µl sample, 10 mg/ml BSA treated gold. -4 offset, 4 sec blot time, 1s drain. Ethane/propane mix. Grid: Quantifoil Cu 2/2 Finder. 10 nm gold beads.
ycw2013-12-21-4_slicer8609.jpg: Myxococcus Xanthus . pili ycw2013-12-21-4_slicer8611.jpg: Myxococcus Xanthus . nested vesicle
Attached Files
20131221_YWC063_10004.mrc, Tilt Series (Pixel Size 0.39 nm), 3.7 GB
20131221_YWC063_10004_part131_5.rec, Reconstruction (Pixel Size 1.56 nm), 712.7 MB
Files
keyimg_ycw2013-12-21-4.jpg
Files
(4.5 GB)
| Name | Size | |
|---|---|---|
| 3.7 GB | (external) Download | |
| 712.7 MB | (external) Download | |
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md5:709da0f00952dde54c09b8ab896b5e21
|
1.1 MB | Preview Download |
|
md5:a876a1328c666700835f460df7aa4ba4
|
25.2 MB | Preview Download |
|
md5:c53ede0ccdb102099bff4e2fa49f609a
|
301.7 kB | Preview Download |
|
md5:e5a7eeafd0dea6cc9068a7f2b83e2f0c
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264.9 kB | Preview Download |
Additional details
Identifiers
- DOI
- 10.22002/k7yb3-yev02
- TILT_SERIES_ID
- ycw2013-12-21-4
Funding
- NIH
- HHMI
- Beckman Institute
- Gordon and Betty Moore Foundation
- Agouron Institute
- John Templeton Foundation
Dates
- Collected
-
2013-12-21
- Created
-
2013-12-21
- Updated
-
2014-07-28