V. cholerae JAS290 delta-tcpS
Description
Technical info
Tilt Series Date: 2016-08-07
Data Taken By: Yi-Wei Chang
Species / Specimen: Vibrio cholerae
Strain: O395-N1
Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 140.0 eV/Ų, defocus: -6.0 μm, magnification: 27500x.
Microscope: Caltech Polara
Acquisition Software: UCSFTomo
Upload Method: pipeline
Processing Software Used: Raptor
Collaborators and Roles: Strain from Gabriela Kovacikova
Purification / Growth Conditions / Treatment: O/N cultures of Vibrio cholerae O395 N1 pMT5 grown in LB, 1 mM IPTG and 100 ug/mL ampicillin.
Sample Preparation: Vitrobot (MK iv) settings: 100% humidity, 3.5µl sample, 10 mg/ml BSA treated gold. Blot force 6, blot time 3 sec. Wait time 0 sec. Drain time 1 sec. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200.
Attached Files
20160807_YWC217_10040.mrc, Tilt Series (Pixel Size 0.39 nm), 3.4 GB
20160807_YWC217_10040_part121_2.rec, Reconstruction (Pixel Size 1.56 nm), 534.5 MB
Files
keyimg_ycw2016-08-07-40.jpg
Additional details
Identifiers
- DOI
- 10.22002/kcgc4-1p948
- TILT_SERIES_ID
- ycw2016-08-07-40
Funding
- NIH
- HHMI
- Beckman Institute
- Gordon and Betty Moore Foundation
- Agouron Institute
- John Templeton Foundation
Dates
- Collected
-
2016-08-07
- Created
-
2016-08-08
- Updated
-
2016-08-18