Caltech Tomography Archive
Published 2015 | Version v1
Dataset Open

CLEM of TRIM5a-YFP in HeLa cells

Creators

  • 1. ROR icon California Institute of Technology

Contributors

Researcher:

Description

HeLa cell line 25 expressing stable TRIM5a-YFP. Correlated light electron microscopy. Concentration of 200 nm red fluorescent beads 1/300. 20 nm gold highly concentrated. Manual blotting with Whatman 40. Fibronectin coated with 0.25 mg/ml diluted with PBS

Technical info

Tilt Series Date: 2015-01-21

Data Taken By: Stephen Carter

Species / Specimen: Hela cells expressing TRIM5a-YFP

Strain:

Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 234.0 eV/Ų, defocus: -10.0 μm, magnification: 18000x.

Microscope: Caltech Polara

Acquisition Software: Undefined

Upload Method: pipeline

Processing Software Used: Raptor

Collaborators and Roles: Tom Hope

Purification / Growth Conditions / Treatment: HeLa cells grown on gold London finder grids for one day. Grids coated with human fibronectin 0.25 mg/ml and 200 nm red fluorescent beads. Red beads cluster.

Attached Files

tomgraphytest5.mrc, Tilt Series (Pixel Size 0.6 nm), 3.4 GB

tomgraphytest5_part121_26.rec, Reconstruction (Pixel Size 2.4 nm), 445.4 MB

Files

keyimg_scd2015-01-21-6.jpg

Files (3.9 GB)

Name Size
md5:53cc0f2631f0349265b412cfc7b6e0e0
1.1 MB Preview Download
md5:a44a2949351e8ed5138b8a73f21528bd
15.6 MB Preview Download
3.4 GB (external) Download
445.4 MB (external) Download

Additional details

Identifiers

DOI
10.22002/ke7en-12976
TILT_SERIES_ID
scd2015-01-21-6

Funding

NIH
HHMI
Beckman Institute
Gordon and Betty Moore Foundation
Agouron Institute
John Templeton Foundation

Dates

Collected
2015-01-21
Created
2015-01-21
Updated
2015-05-07