Azospirillum delta Operon1 and 4

Description

Technical info

Tilt Series Date: 2015-04-15

Data Taken By: Ariane Briegel

Species / Specimen: Azospirillum brasilense

Strain:

Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 160.0 eV/Ų, defocus: 8.0 μm, magnification: 34000x.

Microscope: Caltech Polara

Acquisition Software: UCSFTomo

Upload Method: pipeline

Processing Software Used: Raptor

Collaborators and Roles: Gladys Alexandre, University of Tennessee

Purification / Growth Conditions / Treatment: Grow overnight in 5 mL MMAB+N + 200 μg/mL Ampicillin at RT (on bench) without shaking- Spin down 10' at 3,500 x g- Resuspend pellet in 5 mL MMAB-N- Spin down 10' at 3,500 x g- Resuspend pellet in 5 mL MMAB-N + 200 μg/mL Ampicillin- Leave at RT (on bench) without shaking overnight

Attached Files

AzoDEltoOp1Op4April0015.mrc, Tilt Series (Pixel Size 0.32 nm), 3.4 GB

AzoDEltoOp1Op4April0015_full.rec, Reconstruction (Pixel Size 1.28 nm), 712.7 MB