Hyphomonas AR46
Contributors
Researcher:
Description
Technical info
Tilt Series Date: 2012-09-07
Data Taken By: Yi-Wei Chang
Species / Specimen: Hyphomonas neptunium
Strain: ATCC 15444
Tilt Series Settings: Single Axis, tilt range: (-65.0°, 65.0°), step: 1.0°, constant angular increment, dosage: 100.0 eV/Ų, defocus: -10.0 μm, magnification: 22500x.
Acquisition Software: UCSFTomo
Upload Method: pipeline
Processing Software Used: Raptor
Collaborators and Roles: Strain from Martin Thanbichler
Purification / Growth Conditions / Treatment: Cells grown overnight in Marine Broth (MB) medium at 30 degrees without antibiotics. OD600=0.6
Sample Preparation: Vitrobot settings: 100% humidity, 3µl sample, 10 nm BSA treated gold. -3 offset, 4 sec blot time, no drain. Ethane/propane mix.
Attached Files
20120907_AR46_10024.mrc, Tilt Series (Pixel Size 1.0 nm), 925.0 MB
20120907_AR46_10024_part132_1.rec, Reconstruction (Pixel Size 2.0 nm), 262.8 MB
Files
keyimg_ycw2012-09-07-24.jpg
Additional details
Identifiers
- DOI
- 10.22002/km4zj-tjv49
- TILT_SERIES_ID
- ycw2012-09-07-24
Funding
- NIH
- HHMI
- Beckman Institute
- Gordon and Betty Moore Foundation
- Agouron Institute
- John Templeton Foundation
Dates
- Collected
-
2012-09-07
- Created
-
2012-09-08
- Updated
-
2012-09-13