Published 2015
| Version v1
Dataset
Open
Dflg
Description
This is a flagellum mutant from Sahand (ATP connected) grown in serum bottle. We saw with perfusion setup that the same mutant makes nanowires. So I went back and imaged the serum bottle version just to make sure that it doesnt have nanowires.
Technical info
Tilt Series Date: 2015-08-20
Data Taken By: Poorna Subramanian
Species / Specimen: Shewanella oneidensis
Strain: MR-1
Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 2.0°, constant angular increment, dosage: 150.0 eV/Ų, defocus: -8.0 μm, magnification: 18000x.
Microscope: Caltech Polara
Acquisition Software: UCSFTomo
Upload Method: pipeline
Processing Software Used: Raptor
Collaborators and Roles: Moh and Sahand
Sample Preparation: same as before
Attached Files
dflgwithnanowires_atlas10004_full.rec, Reconstruction (Pixel Size 2.4 nm), 445.4 MB
dflgwithnanowires_atlas10004.mrc, Tilt Series (Pixel Size 0.6 nm), 1.7 GB
Files
keyimg_psa2015-08-20-4.jpg
Additional details
Identifiers
- DOI
- 10.22002/km59v-pvc87
- TILT_SERIES_ID
- psa2015-08-20-4
Funding
- NIH
- HHMI
- Beckman Institute
- Gordon and Betty Moore Foundation
- Agouron Institute
- John Templeton Foundation
Dates
- Collected
-
2015-08-20
- Created
-
2015-08-28
- Updated
-
2016-09-07