Published 2015 | Version v1
Dataset Open

COS7 CHMP4B+VPS4DN

  • 1. ROR icon California Institute of Technology

Contributors

Description

Cos7 cells transfected with CHMP4B & VPS4DN constructs

Technical info

Tilt Series Date: 2015-03-08

Data Taken By: Cora Woodward

Species / Specimen: Cercopithecus aethiops (monkey, African green)

Strain: COS-7 ATCC CRL-1651

Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 150.0 eV/Ų, defocus: -6.0 μm, magnification: 22500x.

Acquisition Software: UCSFTomo

Upload Method: pipeline

Processing Software Used: Raptor

Collaborators and Roles: Constructs provided by Wes Sundquist. Cells transfected, frozen and imaged at Caltech by Cora Woodward

Purification / Growth Conditions / Treatment: whole cells were grown on grids and transfected with Lipofectamine

Sample Preparation: blotting

Attached Files

15mar08_C301-1_AT3BT1_0008.mrc, Tilt Series (Pixel Size 0.5 nm), 3.4 GB

15mar08_C301-1_AT3BT1_0008_full.rec, Reconstruction (Pixel Size 2.0 nm), 623.6 MB

Files

keyimg_cwl2015-03-08-16.jpg

Files (4.1 GB)

Name Size
3.4 GB (external) Download
623.6 MB (external) Download
md5:6f9019b28a37d707a2f175ce876fc122
1.1 MB Preview Download
md5:5263c98504f52f8b1fb6dc0a1a12b555
22.7 MB Preview Download

Additional details

Identifiers

DOI
10.22002/kmm9w-8r786
TILT_SERIES_ID
cwl2015-03-08-16

Funding

NIH
HHMI
Beckman Institute
Gordon and Betty Moore Foundation
Agouron Institute
John Templeton Foundation

Dates

Collected
2015-03-08
Created
2015-03-09
Updated
2019-03-07