Published 2012
| Version v1
Dataset
Open
CheA-Ge8-L2
Description
This strain lacks a chromosomal CheA, and carries plasmid pj207 containing a CheA with a trimer of the silver peptide in linker region two.
Technical info
Tilt Series Date: 2012-03-22
Data Taken By: Ariane Briegel
Species / Specimen: Escherichia coli
Strain: RP 437
Tilt Series Settings: Single Axis, tilt range: (-63.0°, 63.0°), step: 1°, constant angular increment, dosage: 160.0 eV/Ų, defocus: -10.0 μm, magnification: 34000x.
Acquisition Software: UCSFTomo
Upload Method: pipeline
Processing Software Used: Raptor
Collaborators and Roles: Dan Feldheim and Carly Carter
Purification / Growth Conditions / Treatment: grown in TB plus Kanamycin, induced with 14nM IPTG for one hour, penicillin treated for an additional hour
Attached Files
CheAmutantpart20009.mrc, Tilt Series (Pixel Size 0.644 nm), 1.1 GB
CheAmutantpart20009_part128_11.rec, Reconstruction (Pixel Size 1.288 nm), 516.1 MB
Files
keyimg_aba2012-03-22-18.jpg
Additional details
Identifiers
- DOI
- 10.22002/knfdh-5zm36
- TILT_SERIES_ID
- aba2012-03-22-18
Funding
- NIH
- HHMI
- Beckman Institute
- Gordon and Betty Moore Foundation
- Agouron Institute
- John Templeton Foundation
Dates
- Collected
-
2012-03-22
- Created
-
2012-03-23
- Updated
-
2012-03-29