E. coli strain Br/H 266
Description
Technical info
Tilt Series Date: 2004-05-14
Data Taken By: Gavin Murphy
Species / Specimen: Escherichia coli
Strain: MG1655
Tilt Series Settings: Single Axis, tilt range: (-65.0°, 65.0°), step: 0.5°, constant angular increment, dosage: 110.0 eV/Ų, defocus: -8.0 μm, magnification: 0x.
Acquisition Software: UCSF tomo
Upload Method: rundir
Processing Software Used: imod, bsoft
Collaborators and Roles: Conrad Woldringh in Amsterdam, the Netherlands provided the strain; Gavin Murphy collected and processed the data.
Purification / Growth Conditions / Treatment: E. coli Br/H 266. Cells grew slowly, so cells were grown to a high density, probably to stationary phase, then centrifuged.
Sample Preparation: quantifoil grid, 10nm gold, 100% humidity...
Attached Files
S46G2_2_4nm8d220e_SingleTilt.mrc, Tilt Series (Pixel Size 1.2 nm), 2.1 GB
E2_040514_Bin2.mrc, Reconstruction (Pixel Size 0.0 nm), 549.5 MB
Files
keyimg_gme2004-05-14-2.jpg
Additional details
Identifiers
- DOI
- 10.22002/ktvm7-2vp05
- TILT_SERIES_ID
- gme2004-05-14-2
Funding
- NIH
- HHMI
- Beckman Institute
- Gordon and Betty Moore Foundation
- Agouron Institute
- John Templeton Foundation
Dates
- Collected
-
2004-05-14
- Created
-
2008-06-18
- Updated
-
2008-06-11