Hylemonella + Bdellovibrio

Description

Technical info

Tilt Series Date: 2013-01-03

Data Taken By: Yi-Wei Chang

Species / Specimen: Hylemonella gracilis

Strain: ATCC 19624

Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 1.5°, constant angular increment, dosage: 100.0 eV/Ų, defocus: -10.0 μm, magnification: 27500x.

Acquisition Software: UCSFTomo

Upload Method: pipeline

Processing Software Used: UCSFTomo

Purification / Growth Conditions / Treatment: Hylemonella grown 48 hr in Broth 233 at 26°C without antibiotics, Final OD600 < 0.1, incubated with attack-phase Bdellovibrio for 3 days, Spun down by 1000 rcf for 5 min and concentrated by ~10x for plunge freezing

Sample Preparation: Vitrobot setting: 100% humidity, 4 ul sample, 10 nm BSA treated gold, -3 offset, 4 s blot time, no drain, ethane/propane mix

ycw2013-01-03-26_slicer6065.jpg: membrane irregularity ycw2013-01-03-26_slicer6066.jpg: nucleoid

Attached Files

Bdel_Hyle_0020014.mrc, Tilt Series (Pixel Size 0.4 nm), 2.3 GB

Bdel_Hyle_0020014_full.rec, Reconstruction (Pixel Size 1.6 nm), 441.7 MB