Images from "HCR spectral imaging: 10-plex, quantitative, high-resolution RNA and protein imaging in highly autofluorescent samples" suitable for testing out the Unmix 1.0 and Dot Detection 2.0 software packages

Description

These image files provide raw data from the paper entitled "HCR spectral imaging: 10-plex, quantitative, high-resolution RNA and protein imaging in highly autofluorescent samples" suitable for testing out the Unmix 1.0 and Dot Detection 2.0 software packages as follows:

• File 1: zebrafish-10-plex.lif
  • 10-plex RNA imaging in a whole-mount zebrafish embryo (Fig. 2 and Figs S1-S8).
  • Can be used to test out the Unmix 1.0 linear unmixing software package available from Molecular Technologies (MT; www.moleculartechnologies.org), a non-profit academic resource within the Beckman Institute at Caltech.
• File 2: zebrafish-10-plex-matrix.sdm
  • Unmixing matrix coefficients derived from the Leica LasX software for the analysis of File 1.
  • Can be used to test out the Unmix 1.0 linear unmixing software package available from MT.
• File 3: mouse-brain-10-plex.lif
  • 10-plex RNA and protein imaging in a fresh-frozen mouse brain section (Fig. 5 and Figs S22-S33).
  • Can be used to test out the Unmix 1.0 linear unmixing software package available from MT.
• File 4: mouse-brain-10-plex-matrix.sdm
  • Unmixing matrix coefficients derived from the Leica LasX software for the analysis of File 3.
  • Can be used to test out the Unmix 1.0 linear unmixing software package available from MT.
• File 5: dHCR-10-plex.lif
  • dHCR imaging: digital mRNA absolute quantitation in an anatomical context using 10-plex spectral imaging with linear unmixing (Fig. 4 and Figs S20, S21).
  • Can be used to test out the Dot Detection 2.0 software package (available from MT) for detection and colocalization of single-molecule dots in HCR RNA-FISH images.
• File 6: dHCR-10-plex-matrix.sdm
  • Unmixing matrix coefficients derived from the Leica LasX software for the analysis of File 5.

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