Shewanella MR1_Iron cores_Chemos

Description

Technical info

Tilt Series Date: 2018-08-09

Data Taken By: Mohammed Kaplan

Species / Specimen: Shewanella oneidensis

Strain: MR-1

Tilt Series Settings: Single Axis, tilt range: (-50.0°, 50.0°), step: 2°, constant angular increment, dosage: 150.0 eV/Ų, defocus: -9.0 μm, magnification: 27500x.

Microscope: Caltech Polara

Acquisition Software: Serial EM

Upload Method: pipeline

Processing Software Used: Raptor

Collaborators and Roles: These are Shewanella oneidensis MR1 prepared by Sahand (USC) by Chemostat method grown for 2 days and chemically fixed. I (Mohammed) imaged these cells on the Polara to see if the have the iron cores in order to send them to Israel (Sharon Wolf) to perform EDX and confirm the components of the iron cores.

Purification / Growth Conditions / Treatment: Chemostat 2 days growth and chemically fixed. The samples were prepared by Sahand (USC).

Sample Preparation: I prepared these samples by blot force 6, blot time 3s and drain time 1. I also used the Bdellovibrio conditions (force -5, time 4 s). 10 nm Gold nanoparticles were added.

Attached Files

SheweTilt33.mrc, Tilt Series (Pixel Size 0.39 nm), 1.4 GB

SheweTilt33_full.rec, Reconstruction (Pixel Size 1.56 nm), 444.5 MB