Pseudomonas aeruginosa_hypo/-N
Contributors
Researcher:
Description
Technical info
Tilt Series Date: 2017-06-19
Data Taken By: Debnath Ghosal
Species / Specimen: Pseudomonas aeruginosa
Strain:
Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 1.5°, constant angular increment, dosage: 160.0 eV/Ų, defocus: -6.0 μm, magnification: 27500x.
Microscope: Caltech Polara
Acquisition Software: UCSFTomo
Upload Method: pipeline
Processing Software Used: Raptor
Collaborators and Roles: Lisa Racki made samples
Purification / Growth Conditions / Treatment: Cells were concentrated to OD~3 and mixed with 4 nm gold
Sample Preparation: Vitrobot: 100% humidity, blot time 4S, drain time 1 sec, Wait time 0, blot force 6
Attached Files
Pa_20170619_N12c0003_part77_38.rec, Reconstruction (Pixel Size 1.56 nm), 445.4 MB
Pa_20170619_N12c0003.mrc, Tilt Series (Pixel Size 0.39 nm), 2.2 GB
Files
keyimg_dga2017-06-19-46.jpg
Additional details
Identifiers
- DOI
- 10.22002/m7x2b-skj10
- TILT_SERIES_ID
- dga2017-06-19-46
Funding
- NIH
- HHMI
- Beckman Institute
- Gordon and Betty Moore Foundation
- Agouron Institute
- John Templeton Foundation
Dates
- Collected
-
2017-06-19
- Created
-
2017-06-21
- Updated
-
2017-08-17