C. crescentus with ParM plasmid
Description
Technical info
Tilt Series Date: 2005-06-08
Data Taken By: Gavin Murphy
Species / Specimen: Caulobacter crescentus
Strain: NA1000
Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 0.7°, constant angular increment, dosage: 120.0 eV/Ų, defocus: -10.0 μm, magnification: 0x.
Acquisition Software: UCSF tomo
Upload Method: rundir
Processing Software Used: imod, bsoft
Collaborators and Roles: R. Jensen probably gave me the ParM plasmid which was used to transform the Caulobacter, or the other Dane; Data collected and processed by Gavin Murphy
Purification / Growth Conditions / Treatment: Cells were probably grown to exp. phase, then probably centrifuged. They had the ParM plasmid therein.
Sample Preparation: quantifoil grid, 10nm gold, 100% humidity...
Attached Files
C1_S424G2_7s10d120e18k.mrc, Tilt Series (Pixel Size 1.2 nm), 1.5 GB
C1Bin2rec.mrc, Reconstruction (Pixel Size 0.0 nm), 637.5 MB
Files
keyimg_gme2005-06-08-1.jpg
Additional details
Identifiers
- DOI
- 10.22002/m8ess-ffr15
- TILT_SERIES_ID
- gme2005-06-08-1
Funding
- NIH
- HHMI
- Beckman Institute
- Gordon and Betty Moore Foundation
- Agouron Institute
- John Templeton Foundation
Dates
- Collected
-
2005-06-08
- Created
-
2008-06-18
- Updated
-
2008-06-11