Published 2015 | Version v1
Dataset Open

Planktonic cell of Proteus

Creators

  • 1. ROR icon California Institute of Technology

Description

lanktonic cell, PG layer, Proteus mirabillis

Technical info

Tilt Series Date: 2015-12-07

Data Taken By: Qing Yao

Species / Specimen: Proteus mirabilis

Strain: HI4320

Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 180.0 eV/Ų, defocus: -8.0 μm, magnification: 27500x.

Microscope: Caltech Polara

Acquisition Software: UCSF Tomo

Upload Method: pipeline

Processing Software Used: Raptor

Collaborators and Roles: Douglas B. Weibel lab, Wisconsin-Madison university

Purification / Growth Conditions / Treatment: OD=6, 4 ul loading. blotting condition 6 force/ 3 time/ 1 dry time, QUANTIFOIL® – Holey Carbon Films 200 mesh

Sample Preparation: OD=6, 4 ul loading. blotting condition 6 force/ 3 time/ 1 dry time, QUANTIFOIL® – Holey Carbon Films 200 mesh

Attached Files

atlas10008_full.rec, Reconstruction (Pixel Size 1.56 nm), 445.4 MB

atlas10008.mrc, Tilt Series (Pixel Size 0.39 nm), 3.4 GB

Files

keyimg_qya2015-12-07-8.jpg

Files (3.9 GB)

Name Size
3.4 GB (external) Download
445.4 MB (external) Download
md5:35548596994bb91196ca84005cfbe41c
1.1 MB Preview Download
md5:7d9650b449a3f324ba020b1147f0ba68
15.1 MB Preview Download
md5:f92ec1b82124734d8a3db49768f652b8
413.1 kB Preview Download
md5:3d2d539325fa7e47f74cb0245ca69ca4
309.5 kB Preview Download

Additional details

Identifiers

DOI
10.22002/m8g59-h0q60
TILT_SERIES_ID
qya2015-12-07-8

Funding

NIH
HHMI
Beckman Institute
Gordon and Betty Moore Foundation
Agouron Institute
John Templeton Foundation

Dates

Collected
2015-12-07
Created
2015-12-08
Updated
2022-10-12