Published 2015
| Version v1
Dataset
Open
dmsh
Description
This is a mutant in which Msh (Mannose-sensitive hemagglutinin), a type 4 pili in Shewanella has been knocked down. This was done to test whether the pili I see are msh or pilsystem. Turns out, what I see in wt is msh because in the msh knockdown they are absent and pil knockdown they are present. Msh H-Q has been knocked down. This is the complete biosynthetic machinery.
Technical info
Tilt Series Date: 2015-07-08
Data Taken By: Poorna Subramanian
Species / Specimen: Shewanella oneidensis
Strain: MR-1
Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 150.0 eV/Ų, defocus: -8.0 μm, magnification: 22500x.
Microscope: Caltech Polara
Acquisition Software: UCSFTomo
Upload Method: pipeline
Processing Software Used: Raptor
Collaborators and Roles: Moh and Sahand, USC
Purification / Growth Conditions / Treatment: This was grown in serum bottles.
Sample Preparation: blotting conditions - 6,3. drain time 1s.
Attached Files
atlas3_dmsh0009.mrc, Tilt Series (Pixel Size 0.48 nm), 3.4 GB
atlas3_dmsh0009_full.rec, Reconstruction (Pixel Size 1.92 nm), 445.4 MB
Files
keyimg_psa2015-07-08-9.jpg
Additional details
Identifiers
- DOI
- 10.22002/m980x-6gt59
- TILT_SERIES_ID
- psa2015-07-08-9
Funding
- NIH
- HHMI
- Beckman Institute
- Gordon and Betty Moore Foundation
- Agouron Institute
- John Templeton Foundation
Dates
- Collected
-
2015-07-08
- Created
-
2015-07-08
- Updated
-
2015-07-16