Planktonic cell of Proteus
Description
Technical info
Tilt Series Date: 2016-06-18
Data Taken By: Qing Yao
Species / Specimen: Proteus mirabilis
Strain: HI4320
Tilt Series Settings: Single Axis, tilt range: (-55.0°, 55.0°), step: 1.0°, constant angular increment, dosage: 190.0 eV/Ų, defocus: -10.0 μm, magnification: 22500x.
Microscope: Caltech Polara
Acquisition Software: UCSFTomo
Upload Method: pipeline
Processing Software Used: UCSFtomo
Collaborators and Roles: Douglas B. Weibel lab, Wisconsin-Madison university
Purification / Growth Conditions / Treatment: OD=6, 4 ul loading. blotting condition 6s force/ 3s time/ 1s dry time, QUANTIFOIL® – Holey Carbon Films 200 mesh
Sample Preparation: single tilt. constant angular increment, step: 1.0. tilt range: (-60, 60). dosage: 195/A2. defocus: -10um. magnification: 22500.
Attached Files
square50007.mrc, Tilt Series (Pixel Size 0.48 nm), 3.2 GB
square50007_part111_3.rec, Reconstruction (Pixel Size 1.92 nm), 311.8 MB
Files
keyimg_qya2016-06-18-27.jpg
Additional details
Identifiers
- DOI
- 10.22002/m9ybk-xfn09
- TILT_SERIES_ID
- qya2016-06-18-27
Funding
- NIH
- HHMI
- Beckman Institute
- Gordon and Betty Moore Foundation
- Agouron Institute
- John Templeton Foundation
Dates
- Collected
-
2016-06-18
- Created
-
2016-06-20
- Updated
-
2022-10-12