Myxococcus xanthus PilP-sfGFP
Contributors
Researcher:
Description
Technical info
Tilt Series Date: 2014-03-08
Data Taken By: Yi-Wei Chang
Species / Specimen: Myxococcus xanthus
Strain:
Tilt Series Settings: Single Axis, tilt range: (-65.0°, 65.0°), step: 1.0°, constant angular increment, dosage: 200.0 eV/Ų, defocus: -8.0 μm, magnification: 26000x.
Microscope: Janelia Titan
Acquisition Software: UCSFTomo
Upload Method: pipeline
Processing Software Used: Raptor
Collaborators and Roles: Strain form Lotte Sogaard-Andersen
Purification / Growth Conditions / Treatment: Cells grown 48 hr in shaking CTT medium at 30 degrees without antibiotics. OD600=0.8
Sample Preparation: Vitrobot settings: 100% humidity, 3µl sample, 10 mg/ml BSA treated gold. -3.5 offset, 4 sec blot time, no drain. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200.
ycw2014-03-08-37_slicer7723.jpg: pili in Myxococcus Xanthus ycw2014-03-08-37_slicer7725.jpg: filaments in Myxococcus Xanthus ycw2014-03-08-37_slicer7728.jpg: unknown
Attached Files
20140308_YWC093_10001.mrc, Tilt Series (Pixel Size 0.42 nm), 3.6 GB
20140308_YWC093_10001_part131_1.rec, Reconstruction (Pixel Size 1.68 nm), 688.9 MB
Files
keyimg_ycw2014-03-08-37.jpg
Files
(4.3 GB)
| Name | Size | |
|---|---|---|
| 3.6 GB | (external) Download | |
| 688.9 MB | (external) Download | |
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md5:b6a561e506d9018e80504f6baa94708c
|
998.5 kB | Preview Download |
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md5:86ac58133c2c811991f4fe39f0d28288
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25.4 MB | Preview Download |
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md5:0fe65189ad9ecab5510fa9703c615348
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273.5 kB | Preview Download |
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md5:0f7dc45e0f835a9de3555255cdff6ed5
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390.1 kB | Preview Download |
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md5:d8486e9a921be9a8ac5ec95f5862d1a2
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402.8 kB | Preview Download |
Additional details
Identifiers
- DOI
- 10.22002/md4nf-bv791
- TILT_SERIES_ID
- ycw2014-03-08-37
Funding
- NIH
- HHMI
- Beckman Institute
- Gordon and Betty Moore Foundation
- Agouron Institute
- John Templeton Foundation
Dates
- Collected
-
2014-03-08
- Created
-
2014-03-11
- Updated
-
2014-07-21